تهیه فیلم نانوکامپوزیت کیتوزان - نانوذرّات اکسید روی - اسانس آویشن و بررسی ویژگی‌های مکانیکی، ساختاری و ضدمیکروبی آن

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه آموزشی صنایع غذایی، دانشکده کشاورزی، واحد صفا دشت، دانشگاه آزاد اسلامی، صفا دشت، ایران.

2 استادیار، دکتری تخصصی، گروه آموزشی صنایع غذایی، دانشکده کشاورزی، واحد صفا دشت، دانشگاه آزاداسلامی، صفا دشت، ایران

3 دانش آموخته کارشناسی ارشد- گروه علوم و صنایع غذایی- دانشکده کشاورزی- مؤسسه آموزش عالی آفاق

چکیده

بسته‌بندی‌های زیست‌تخریب‌پذیر به دلیل ویژگی‌های دوستدار محیط ‌زیست بودن جایگزین مناسبی برای فیلم‌های سنتزی در صنعت بسته‌بندی می‌باشند. در این پژوهش از نانو ذرّات اکسید روی و اسانس آویشن برای بهبود خصوصیات ساختاری کیتوزان استفاده شد که بدین منظور نانو کامپوزیت کیتوزان- نانو اکسید روی (03/0 -01/0٪) – اسانس آویشن(25/0- 5/0٪) تولید شد. تفاوت نمونه ها با فیلم شاهد با هدف تولید بسته‌بندی ضد باکتری و زیست‌تخریب‌پذیر است، خصوصیات ساختاری با استفاده از روش های طیف‌سنجی مادون‌قرمز، پراش اشعه ایکس، بررسی گردیدند و مورفولوژی ذرّات سنتز شده با استفاده از روش میکروسکوپ الکترونی روبشی تعیین شد. نتایج به‌دست‌آمده از بررسی خصوصیات ساختاری نشان داد نانو ذرّات اکسید روی سنتز شده در ماتریکس پلیمری کیتوزان در ابعاد نانومتری می‌باشند. بر اساس نتایج به دست آمده با افزایش غلظت نانواکسید روی و اسانس آویشن، حلالیت آبی و نفوذپذیری به بخارآب فیلم‌ها 70٪ کاهش یافت. برهم‌کنش بین کیتوزان، نانواکسید روی و اسانس آویشن توسط الگوی طیف‌سنجی مادون‌قرمز و پراش اشعه ایکس تأیید شد. یافته های این پژوهش عاملی مهم برای گسترش استفاده از فیلم های زیست تخریب با بهبود خصوصیات عملکردی آنها توسط نانوذرّات می باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Preparation of Chitosan- Zinc Oxide- Thyme Essential Oil Nanocomposite Film, Study on Mechanical, Structural and Antimicrobial Properties

نویسندگان [English]

  • Zahra Shabahang 1
  • sepideh bahrami 2
  • Behzad Mohammadi 3
1 Department of Conservation Food Science and Technology, Safadasht Branch, Islamic Azad University, Safadasht, Iran
2 Department of Conservation Food Science and Technology, Islamic Azad University, Safadasht, Iran
3 Graduate master, Department of Food Science and Technology, Faculty of Agriculture, Institute of higher education Afagh
چکیده [English]

Biodegradable packaging is a good alternative to synthetic film in the packaging industry because of its environmental-friendly. In this study, zinc oxide nanoparticles and thyme oil were used to enhance the structural properties of chitosan, which were produced for the purpose of chitosan - zinc oxide (0.01- 0.03%)  - thyme essential oil (0.25- 0.5%) nanocomposite. The differences between the synthesized samples with control film (blank) are for production of antibacterial and biodegradable packaging, the structural properties were investigated by infrared spectroscopy and X-ray diffraction, also the morphology of the synthesized particles was investigated by scanning electron microscopy. The results of the structural properties showed that the zinc oxide synthesized in the chitosan polymer matrix were nanoparticles. Based on the results, the water solubility and water vapor permeability of films decreased by 70% with increasing zinc oxide concentration and thyme essential oil. The interaction between chitosan, zinc oxide nanoparticles and thyme essential oil was confirmed by infrared spectroscopy and X-ray diffraction pattern. The findings of this study are an important factor for expanding the use of biodegradation films by improving their functional properties by nanoparticles.

کلیدواژه‌ها [English]

  • chitosan
  • Zinc oxide Nanoparticles
  • Thyme Essential Oil
  • Biodegradability
  • Antibacterial
1. Ghanbarzadeh, B., Almasi, H. (2011). “Physical properties of edible emulsified films based on carboxymethyl cellulose and oleic acid.” International Journal of Biological Macromolecules, 48,44-49.

2. Teng, B , k. G. (2012). Formation and properties of clay-polymer compelexes,” 2nd ed., Vol. 4. Amsterdam: Elsevier, 2012.

3. Luo, Y ., and Wang. Q . (2013). “Recent advances of chitosan and its derivatives for novel applications in food science.” J. Fd. Proc. Bev. 1.13.

4. Je J. and Kim S., (2006) “Chitosan derivatives killed bacteria by disrupting the outer and inner membrane,” Journal of Agricultural Food Chemistry, vol. 54, pp. 6629–6633.

5. Rabea E.I., Badawy M.E.-T., Stevens C.V., Smagghe G., and W. S., (2003). “Chitosan as antimicrobial agent: Applications and mode of action,” Biomacromolecules, vol. 4, pp. 1475–1465.

6. Zeng, L., Jiang, Y.,Ding, Y., Pove, D. (2007). “Investigation into the antibacterial behavior of suspensions of zno nanoparticles.” Journal of nanoparticles research,9, 479-489 .

7. Davidson PM, Zivanovic S. (2003). “The use of natural antimicrobials. In: Food preservation techniques.” Woodhead Publishing Limited and CRC Press. 1st ed. CRC press: Washington; 5-8.

8. Marino, M., Bersani, C., & Comi, G. (2001). “Impedance measurement to study antimicrobial activity of essential oils from Lamiaceae and Compositae.” International Journal of Food Microbiology, 67, 187–195.

9. Pasquini, D., Teixeira, E. M., Curvelo, A.S., Belgasem, M. N., & Dufresne, A. (2008). “Surface esterification of cellulose Fibres: Processing and Characterisation of low – density polyethylene / cellulose fibres composites.” Composites Science & Technology. 68:193-201.

10. Peng, Y., Wu, Y., and Li, Y. (2013). “Development of tea extracts and chitosan composite film for active packaging materials.” International journal of biological macromolecules 59, 282- 289.

11. Mogadas Kia, E., Alizadeh, M., Wrdast, M., Rezazad, M. (2016). “Synthesis of nanoplimate of absorbent surface molecular mold Cholesterol based on magnetic iron-silica particles and functional properties review,” Urmia Medical Journal Twenty-seventh volume, 3, 231-239..

12. Casariego, A., Souza, B., Cerqueira.m M., Texeira, J., Cruz, Diaz, R., and Vicente, A. (2009). “Chitosan0clay films properties as affected by biopolymer and clay micro/nanoparticales concentration.” Food Hydrocolloids 23, 1895- 1902.

13. Abdollahi, M., Alboofetileh, M., Behrooz, R., Rezaei, M., Miraki, R. (2013). “Reducing water sensitivity of alginate bio- nanocomposite film using cellulose nanoparticles.” International journal of biological macromolecules, 54, 166-173.

14. Saadatmand, M., Yazdanshenas, M., Rezaie Zarachi, S., Yousefi Tellouri, B. (2012). “Antimicrobial activity of chitosan-TiO2 nanocomposite and its application on hospital sterilized gas.” Journal of Experimental Sciences. Spring and Summer, 6, 1: 59-62.

15. Qi, L., Xu, Z., Jiang, X., Hu, C., & Zou, X. (2004). “Preparation and antibacterial activity of chitosan nanoparticles.” Carbohydrate research, 339(16), 2693-2700.

16. Abdeen, Z., & Mohammad, S. G. (2013). “Study of the adsorption efficiency of an eco-friendly carbohydrate polymer for contaminated aqueous solution by organophosphorus pesticide.” Open Journal of Organic Polymer Materials, 4(1), 16-28.

17. Li, Y., Jiang, Y., Liu, F., Ren, F., Zhao, G., & Leng, X. (2011). “Fabrication and characterization of TiO 2/whey protein isolate nanocomposite film.” Food Hydrocolloids, 25(5), 1098-1104.

18. Zhang, Y., Kohler, N., & Zhang, M. (2002).“Surface modification of superparamagnetic magnetite nanoparticles and their intracellular uptake.” Biomaterials, 23(7), 1553-1561.

19. Rosanny, S. C., Sarmento, M. V., Nogueira, F. A., Tonholo, J., Mortimer, R. J., Faez, R., & Ribeiro, A. S. (2014). “Enhancing the electrochromic response of polyaniline films by the preparation of hybrid materials based on polyaniline, chitosan and organically modified clay.” RSC Advances, 4(29), 14948-14955.

20. Yavuz, A. G., Uygun, A., & Bhethanabotla, V. R. (2009). “Substituted polyaniline/chitosan composites: Synthesis and characterization.” Carbohydrate Polymers, 75(3), 448-453.

21. Xu, X. H., Ren, G. L., Cheng, J., Liu, Q., Li, D. G., & Chen, Q. (2006). “Self-assembly of polyaniline-grafted chitosan/glucose oxidase nanolayered films for electrochemical biosensor applications.” Journal of materials science, 41(15), 4974-4977.

22. Hong R. Y., Li J. H., Chen L. L., Liu D. Q., Li H. Z., Zheng Y. and Ding J., (2009)“Synthesis, surface modification and photocatalytic property of ZnO nanoparticles”, Powder Technol., 13, pp. 426–432.

23.  Hong R. Y., Chen L. L., Li J. H., Li H. Z., Zheng Y. and Ding J., (2007). “Preparation and application of polystyrenegrafted ZnO nanoparticles”, Polym. Adv. Technol., 18, pp. 901–909.

24. Shafei A, Abou-Okeil A. (2011). “ZnO/carboxymethyl chitosan bionano-composite to impart antibacterial and UV protection for cotton fabric.” Carbohydr Polym;. 83(2):920-5.

25. Roselli M, Finamore A, Garaguso I, Britti MS, Mengheri E. (2003). “Zinc oxide protects cultured enterocytes from the damage induced by Escherichia coli.” J Nutr; 133(12):4077-82.

‏26. Wang, G. H. (1992). “Inhibition and inactivation of five species of foodborne pathogens by chitosan.” Journal of Food Protection, 55 (11), 916–919.

27. Pranoto, Y., Rakshit, S. K., & Salokhe, V. M. (2005). “Enhancing antimicrobial activity of chitosan films by incorporating garlic oil, potassium sorbate and nisin.” LWT, 38, 859–865.

28. Bourtoom, T., & Chinnan, M. S. (2008). “Preparation and properties of rice starch–chitosan blend biodegradable film.” LWT-Food Science and Technology, 41(9), 1633-1641.

29. Garcia, M.A., Pinotti, A., Martino, M.N., and Zaritzky, N.E. (2004). “Characterization of composite hydrocolloid films.” Carbohydate Polymer, 56: 339-345.

30. Rhim, J. W., & Kim, Y. T. (2014). “Biopolymer-based composite packaging materials with nanoparticles.” In Innovations in Food Packaging (Second Edition) (pp. 413-442).

31. Casariego, A., Souza, B., Cerqueira.m M., Texeira, J., Cruz, Diaz, R., and Vicente, A. (2009). “Chitosan/clay films properties as affected by biopolymer and clay micro/nanoparticales concentration.” Food Hydrocolloids 23(7), 1895- 1902.

32. Abdollahi, M., Rezaei, M., & Farzi, G. (2012). “A novel active bionanocomposite film incorporating rosemary essential oil and nanoclay into chitosan.” Journal of Food Engineering, 111(2), 343-350.

33. Rhim, J. W., Hong, S. I., Park, H. M., & Ng, P. K. (2006). “Preparation and characterization of chitosan-based nanocomposite films with antimicrobial activity.” Journal of agricultural and food chemistry, 54(16), 5814-5822.

35. Cosentino, S., Tuberoso, C.I.G., Pisano, B., Satta, M., Mascia, V., Arzedi, E. and Palmas, F. (1999). “In vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils.” Applied Microbiology, 29, 130–135.

36. Rhim, J. W. (2011). “Effect of clay contents on mechanical and water vapor barrier properties of agar-based nanocomposite films.” Carbohydrate polymers, 86(2), 691-699.

37. Chen, M. C., Yeh, G. H., & Chiang, B. H. (1996). “Antimicrobial and physicochemical properties of methylcellulose and chitosan films containing a preservative.” Journal of Food Processing and Preservation, 20, 379-390.